Minimized Cell Usage for Stem Cell-Derived and Primary Cells on an Automated Patch Clamp System

Automated and manual patch clamp data of human induced pluripotent stem cell-derived dopaminergic neurons

Human induced pluripotent stem cells can be differentiated into dopaminergic neurons (Dopa.4U). Dopa.4U neurons expressed voltage-gated NaV and KV channels and showed neuron-like spontaneous electrical activity. In automated patch clamp measurements with suspended Dopa.4U neurons, delayed rectifier K+ current (delayed KV) and rapidly inactivating A-type K+ current (fast KV) were identified. Exa...

Action potential characterization of human induced pluripotent stem cell-derived cardiomyocytes using automated patch-clamp technology.

Recent progress in embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) research led to high-purity preparations of human cardiomyocytes (CMs) differentiated from these two sources-suitable for tissue regeneration, in vitro models of disease, and cardiac safety pharmacology screening. We performed a detailed characterization of the effects of nifedipine, cisapride, and tetrodotoxi...

expression of potassoim channel in stem cell derived-cardiomyocytes evaluated by patch-clamp technique

assessment of potassium current in royan b1 stem cell derived cardiomyocytes by patch-clamp technique

embryonic stem cells are capable of differentiating to variety of cell tissues including cardiomyocytes. this developmental change is accompanied with a great deal of ion channel expression and functions. mouse stem cell derived cardiomyocytes were prepared and separated to yield isolated single cell suspension for cell current recording. in the present study some properties of the k + -current...

Automated patch clamp on mESC-derived cardiomyocytes for cardiotoxicity prediction.

Cardiovascular side effects are critical in drug development and have frequently led to late-stage project terminations or even drug withdrawal from the market. Physiologically relevant and predictive assays for cardiotoxicity are hence strongly demanded by the pharmaceutical industry. To identify a potential impact of test compounds on ventricular repolarization, typically a variety of ion cha...